Fig. 5: Cryo-EM analysis of 20%Q + N-microtubules.
From: Hydrolysis-deficient mosaic microtubules as faithful mimics of the GTP cap
A Histogram of the relative abundance of 12- to 15-protofilament (pf) microtubule specimens observed in cryo-EM datasets (see also Supplementary Table 2). Plus (+) and minus (−) ends are indicated. B, C Cryo-EM structures of undecorated (B) and EB3-CH-decorated (C) 20%Q + N-microtubules. D, E Atomic model building of mosaic microtubules from their corresponding symmetrized maps. Comparison of the Cα traces of two consecutive dimers after model superimposition on the intermediate domain of the α2-tubulin chain. D Comparison between undecorated 20%Q + N-microtubules (magenta) and undecorated WT microtubules (green) (PDB: 7SJ7)32. E Comparison between undecorated 20%Q + N-microtubules (magenta) and CH-EB3-decorated 20%Q + N-microtubules (gray). The dimer rise (next to bottom tubulin dimers) and protofilament twist (at the top) as determined from the C1 maps using RELION are indicated.
