Table 2 Calculated biochemical parameters ± SEM
From: Hydrolysis-deficient mosaic microtubules as faithful mimics of the GTP cap
WT | αE254Q | αE254N | 20%Q + N | |
|---|---|---|---|---|
kon,(app) (µM−1 s−1) | 3.9 ± 0.3 | 6.2 ± 0.3 | 2.6 ± 0.5 | 3.7 ± 0.1 |
koff,(app) (s−1) | 7.0 ± 4.3 | 3.4 ± 1.6 | 11.9 ± 7.5 | 0.9 ± 1.5 |
Lp(app) (µm) | 416 ± 88 | 240 ± 38 | – | 520 ± 31 |
K* (µM−1 s−1 10−5) | 60.0 ± 7.6 | 170.0 ± 5.8 | – | 71.7 ± 5.9 |
mGFP-EB3 Kd (nM) | 39.6 ± 5.8* | 8.3 ± 2.9 | – | 14.0 ± 2.6 |
