Fig. 1: Phenotypic change of VZV gE-specific CD4+ T cells during RZV vaccination. | Nature Communications

Fig. 1: Phenotypic change of VZV gE-specific CD4+ T cells during RZV vaccination.

From: Longitudinal single cell profiling of epitope specific memory CD4+ T cell responses to recombinant zoster vaccine

Fig. 1: Phenotypic change of VZV gE-specific CD4+ T cells during RZV vaccination.The alternative text for this image may have been generated using AI.

Representative FACS analysis on VZV gE-specific CD4+ T cells by surface staining of CD45RA, CXCR5, CXCR3, CCR4, CCR6, CCR7, CD38, PD-1 and ICOS on CD4+CD154+Tetramer (DR0401/gE280-299)+ cells obtained from donor 1 at days 0 (baseline), 14, 60, 74 and 365 post-first dose of Shingrix (second dose was administered after sample collection at day 60). The CD154(PE)+Tetramer(PE-Cy7 or PE-Dazzle 594)+ cells were enriched by anti-PE MicroBeads and MS column then stained with other surface antibodies described above before flow cytometry analyses and sorting.

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