Fig. 3: Spatial omics demonstrate the T-cell interactome across different histomorphic niches in NF2 SWN-related vestibular schwannoma.

A From left to right, IMC visualisation of T-cells across Antoni A and Antoni B regions. Markers used: S100B (purple), Iba1 (red), CD8α (green), CD4 (white) and SMA (cyan). Next, single-cell spatial seaborn maps of all T-cell populations, and co-localisation to Schwann cell or TAM populations with the vascular networks across Antoni A and B regions. Scale bar indicates 100 μm. B Cross-pair correlation functions (PCF) heatmaps showing T-cell interaction partners with all other cell populations; scale bar indicates strength of significant cell pair correlates. C Spatial connectivity plots indicating the significant networks of T-cell populations, by adjacency cell network (ACN) analysis, across Antoni A and Antoni B histomorphic niches, with representative images of each network. Node size (coloured circle) indicates mean abundance for each cell cluster across all ROIs; lines connecting each node show significant cell networks between cell types informed by ACN analysis. Line thickness associates the gr20 value to each significant cell pair, where the thicker the line, the higher the co-localisation of the cell populations. the scale bar indicates strength of connectivity. B, C *p < 0.05, using cross-PCF and ACN statistical analyses, see Methods (n = 13). Source data are provided as a Source Data file.