Table 2 IMC antibody panel

From: Spatial mapping of immune cell environments in NF2-related schwannomatosis vestibular schwannoma

Compartment	Acronym	Antigen	Target	Metal Tag	Dilution
Neoplastic	S100B	S100 calcium-binding protein B	Schwann cells	141Pr	1:200
	SOX10	SRY-related HMG-Box 10	Schwann cells	148Nd	1:100
	PanCK	Pan-cytokeratin	Tumour cells	139La	1:100
Immune
- Pan	CD45	Protein tyrosine phosphatase receptor type C	Blood cells	152Sm	1:50
	TIM-3	T-cell immunoglobulin and mucin-domain containing-3	Immunosuppression	161Dy	1:100
	Granzyme B	–	Cytotoxicity	151Eu	1:100
	CD16	FcγRIII	Immune cells	146Nd	1:100
	CX3CR1	Fractalkine receptor	Immune cell migration	172Yb	1:100
	CD11b	Integrin alpha M	Innate immune cells	149Sm	1:100
	CD11c	Integrin alpha X	Immune cells	154Sm	1:50
- Myeloid	CD14	–	Monocytes/macrophages	144Nd	1:50
	CD163	–	Macrophages	196Pt	1:50
	Iba1	Ionised calcium-binding adaptor molecule 1	Macrophages	115Ln	1:50
	CD74	HLA-II histocompatibility antigen gamma chain	Antigen-presenting cells	166Er	1:100
	HLA-DR	Human leucocyte antigen DR	Antigen-presenting cells	174Yb	1:100
	CD206	Mannose receptor	Macrophages	162Dy	1:100
	CD68	–	Macrophages	198Pt	1:50
- Lymphoid	CD3	CD3	T cells	170Er	1:50
	CD4	–	Helper T cells	156Gd	1:50
	CD8α	CD8 alpha chain	Cytotoxic T cells	175Lu	1:100
	CD45RA	–	Naïve/exhausted T cells	158Gd	1:100
	CD45RO	–	Memory T cells	173Yb	1:100
	TCIRG1	T cell immune regulator 1	T cells	165Ho	1:50
Vasculature	SMA	Smooth muscle actin	Blood vessel structure	89Y	1:100
	CD31	Platelet endothelial cell adhesion molecule 1	Blood vessel endothelium	153Eu	1:100
	vWF	von Willebrand factor	Endothelial cell activation	159Tb	1:100
Miscellaneous	HLA-A	Human leucocyte antigen A	Nucleated cells	142Nd	1:50
	CD235ab	Glycophorin A/B	Erythrocytes	195Pt	1:50
	Vimentin	–	Cell motility and stability	143Nd	1:100
	CAIX	Carbonic anhydrase IX	Hypoxia	169Tm	1:100
	HIF-1α	Hypoxia-inducible factor 1 alpha	Hypoxia	155Gd	1:50
	GLUT1	Glucose transporter 1	Hypoxia and metabolism	163Dy	1:100
	PD-L1	Programme death-ligand 1	Immune suppression	167Er^a	Neat^b
	Ki-67	–	Proliferation	168Er	1:100
	AGTR1	Angiotensin II type 1 receptor	Signalling	164Dy	1:100
	pERK1/2	Phosphorylated extracellular signal-regulated kinase 1/2	Signalling	171Yb	1:100
	MCT4	Monocarboxylate transporter 4	Signalling	176Yb	1:200
	CD44	Hyaluronan receptor	Signalling	150Nd	1:50

^aPD-L1 was detected by a secondary anti-rabbit antibody conjugated with 167Er.
^b100 μL of neat PD-L1 was used for staining. Specific antibody concentration is approximately 1.61μg/mL.

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Table 2 IMC antibody panel

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