Fig. 2: The characterizations of OCPNs and proof-of-concept studies.
From: POSA for fast, amplified and multiplexed protein imaging
a Schematic of FCP-g-DNA amphiphilic polymer synthesis via solid-phase “Click” reaction and the self-assembly of OCPN. The chemical structures of π-conjugated polymers are shown. b normalized UV-vis absorption spectra, (c) fluorescence spectra, (d) DLS data, and TEM images of OCPNs. e Fluorescent images of TOMM20 in HeLa cells employing AF488-labeled secondary antibody (AF488-SA). The anti-TOMM20 primary antibody (PA) was either adopted as is, modified with sulfhydryl group after the TCEP treatment, or modified with BS4. f Fluorescent images of TOMM20 in HeLa cells pre-immunolabelled by PA4-BS4 using PFBT-g-A20 OCPNs, PFBT-g-T20 OCPNs, and PFBT-g-IS4 OCPNs, respectively. g Orthogonal imaging of anti-Ki67 PA2-IS2, anti-Vimentin PA7-BS7, and anti-EGFR PA8-BS8 in HeLa cells using PFBT-g-IS2 OCPNs, PFBT-g-IS7 OCPNs, and PFBT-g-IS8 OCPNs. h Vimentin, Lamin A/C, and mitochondria were simultaneously imaged in HeLa cells using PFBT OCPNs (the green channel) and Cy5-labeled SA (the red channel). i Colocalization analysis between immunofluorescence (IF) and POSA for three proteins from (h) indicates a good correlation (Pearson’s R from 0.79 to 0.95). j Three-color multiplexed imaging of α-tubulin, mitochondria, and Lamin A/C in HeLa cells via POSA. Source data are provided as a Source Data file.
