Fig. 4: Single-cell genotype-to-phenotype analyzes highlights regulators of yeast cell states.

a Distribution of the number of enriched cell states per genotype in control conditions. b Volcano plot shows the cell state enrichment of each mutant in control conditions (wild type is shown in red). Black line indicates the threshold for statistical significance (two-sided Fisher test Benjamini-Hochberg adjusted p-value < 0.05). c Number of genotypes enriched (blue) or depleted (red) per each cell state in each condition. d Protein enrichment network of mutants in control condition enriched in cluster 8 C (odds ratio >1, two-sided Fisher test Benjamini–Hochberg adjusted p-value < 0.05). Nodes are colored according to module enrichment (MDCE, see Methods). e Expression of the pFIT3 reporter (8 C) measured by flow cytometry, reporter expression is shown in the x axis against the side scatter 8SSC) for the indicated strains colored as in (d). Histograms represent density of the x and y axis. Graphs shows a representative experiment. Mean, standard deviation and significance to the wild type (paired t.test) (n = 3). f Representative images of mitochondrial morphology stained by MitoTracker for the indicated strains. Barplots show average of each mitochondrial morphology under control conditions (n = 3, 150 cells/strain). Paired t.test was performed comparing the frequency of tubular morphology between the wild type and mutant strains (pvalues 0,00019 (mss116), 6,42E-05 (mrpl7), 1,30E-05 (snf7), 0,00012 (rim8)). g Protein–protein interaction for genotypes enriched in cell state 9 C colored according to their MDCE. h Levels of Lysine acetylation determined by ChIP at the indicated strains and promoters in control conditions for the indicated promoters. Bars represent the mean and standard deviation of three independent biological replicates (dots). Benjamini-Hochberg corrected paired t.test is shown respect to the wild type (pvalues for the indicated strains (left to right) PHO84; 0.00219, 0.00043, 0.00022, 7.6e-05; VTC1; 0.0126, 0.0126, 0.0126, 0.0097. i Growth curve of the indicated strains in control (CSM pH=5.5) or alkaline conditions (CSM pH=8.8). The bars represent the average and standard deviation between the difference in Lag time between the alkaline media respect to control (n = 3). Benjamini–Hochberg corrected paired t-test is shown respect to the wild type (p-value for the indicated strains (left to right) 0.0171, 0.0002, 0.0012, 0.0082. j Radar plot shows the fitness score across a stressor panel for mutants enriched in specific cell states in control conditions (orange) or a random permutation as a reference (gray). Light gray ribbons show the 95% confidence interval. k Fitness score as in j for mutants enriched in cell states in stress conditions (blue). Source data are provided as a Source Data file.