Fig. 1: Tet2^KO HSPCs are dependent on DOT1L activity.

A Percentage of BFP⁻Tet2^WT or BFP⁻Tet2^KO HPC^HOXB4 cells in competition with BFP⁺Tet2^WT HPC^HOXB4 cells at the indicated time points after starting the experiment. n = 3 experimental replicates. Unpaired t test used for significance. B The percentage of BFP⁻Tet2^KO HPC^HOXB4 cells after treatment with the indicated compounds or DMSO (highlighted in orange) for 14 days. The dashed line represents the percentage of Tet2^KO HPC^HOXB4 in DMSO treatment. Data represent the average of 3 experimental replicates. Representative data from one of 3 independent screens are shown. C Count of Annexin V negative Tet2^KO or Tet2^WT HPC^HOXB4 cells treated with 1 μM of each compound over time normalized to the corresponding untreated sample. n = 3 experimental replicates. Unpaired t test with multiple testing corrections used for significance. D Percentage of Venus⁺Tet2^WT or Venus⁺Tet2^KO HPC^HOXB4 cells in competition with Venus⁻Tet2^WT HPC^HOXB4 cells over time. Both cell types were transduced with the indicated shRNA vectors. n = 3 experimental replicates. Unpaired t test used for significance. E Proportion of CD45.2⁺Tet2^KO or CD45.2⁺Tet2^WT HSPCs in competition with CD45.1⁺Tet2^WT HSPCs after treatment with DMSO or SGC0946 (SGC; 1 μM). Data are normalized to DMSO-treated CD45.2⁺Tet2^WT cells. Unpaired t test used for significance. n = 3 experimental replicates. F Proportion of CD45.2⁺Tet2^KO or CD45.2⁺Tet2^WT HSPCs in competition with CD45.1⁺Tet2^WT HSPCs gated on the transduced BFP⁺ population. Data are normalized to shNT-expressing CD45.2⁺Tet2^WT cells. Unpaired t test used for significance. n = 3 experimental replicates. Data are shown as mean ± SEM. G Number of colonies formed in per 1 × 10⁴ Lin^-Kit⁺ (LK) HSPCs in the presence of DMSO or SGC0946 (SGC; 1 μM). n = 3 experimental replicates. Unpaired t test used for significance. All data shown are mean ± SD unless otherwise indicated. Asterisks indicate statistical significance. Source data are provided as a Source Data file.