Fig. 2: sEV subtypes have similar diameters but different membrane defects. | Nature Communications

Fig. 2: sEV subtypes have similar diameters but different membrane defects.

From: Uptake of small extracellular vesicles by recipient cells is facilitated by paracrine adhesion signaling

Fig. 2: sEV subtypes have similar diameters but different membrane defects.

a Representative magnified view (top) and wide-field view (bottom) of PC-3 cell-derived sEV particles visualized with the negative-stain transmission electron microscope (TEM), selected from 24 independent TEM fields. b sEV diameters were determined using TEM (top), qNANO (middle), and direct stochastic optical reconstruction microscopy (dSTORM) (bottom). Numbers indicate means ± SD. “n” indicates the number of examined sEV particles derived from PC-3 cells. c Size analysis using dSTORM. Typical images of an sEV containing CD9, CD63, and CD81 stained with SaraFluorTM650B (SF650B) via Halo7 and a DMPC liposome containing GM3 conjugated with SF650B at the terminal sialic acid observed by TIRF microscopy. dSTORM images were determined using the Voronoi-based segmentation method (see Methods for details). d Distribution of fluorescence intensities of the C-terminal region of apolipoprotein A-I labeled with 5-carboxytetramethylrhodamine (ApoC-TAMRA) on individual PC-3 cell-derived sEVs containing CD9, CD63, and CD81 labeled with SaraFluorTM650T (SF650T) via Halo7 isolated by ultracentrifugation. “n” indicates the number of examined sEV particles. Statistical analyses were conducted as described in “Statistics and Reproducibility” in the Methods section.

Back to article page