Fig. 2: her1 and her7 RNA counts differentially change in different parts of PSM in each mutant genotype.

a The PSM tissue is divided into single-cell-wide slices and categorized into four groups. Slices ranging from 1 to 10, 11 to 30, 31 to 38, 39 to 56 are designated as groups 1, 2, 3, and 4, respectively. Group 1 covers the tailbud. Group 2 ends at the determination front (light blue line and arrows). Group 3 and 4 are separated based on changes in transcription patterns. b her1 d her7 and f her1 plus her7 (total her) RNA numbers in her1^ci301;her7^hu2526 (dark yellow, N = 2, n = 14), her1^ci301;her7^hu2526;dlc^tw212b (black, N = 2, n = 16), her1^ci301;her7^hu2526;dld^tr233 (purple, N = 3, n = 13), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 2, n = 11) embryos are plotted from posterior to anterior direction. Experimental, and embryo numbers are represented as N, and n respectively. The average RNA number in each slice, along with 2 s.e.m., is represented by dots and error bars. Light gray dashed lines show the boundaries between four expression domains in the PSM. c her1 e her7 and g her1 plus her7 (total her) RNA numbers are compared across four genotypes, by averaging all embryos in each group, as indicated in (a). c e, g In Group 1, RNA numbers coming from 10 slices in her1^ci301;her7^hu2526 (dark yellow, N = 2, n = 14, s = 234), her1^ci301;her7^hu2526;dlc^tw212b (black, N = 2, n = 16, s = 288), her1^ci301;her7^hu2526;dld^tr233 (purple, N = 3, n = 13, s = 221), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 2, n = 11, s = 181). In Group 2, RNA numbers coming from 20 slices in her1^ci301;her7^hu2526 (dark yellow, N = 2, n = 14, s = 560), her1^ci301;her7^hu2526;dlc^tw212b (black, N = 2, n = 16, s = 636), her1^ci301;her7^hu2526;dld^tr233 (purple, N = 3, n = 13, s = 514), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 2, n = 11, s = 420). In Group 3, RNA numbers coming from 8 slices in her1^ci301;her7^hu2526 (dark yellow, N = 2, n = 14, s = 224), her1^ci301;her7^hu2526;dlc^tw212b (black, N = 2, n = 16, s = 256), her1^ci301;her7^hu2526;dld^tr233 (purple, N = 3, n = 13, s = 206), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 2, n = 11, s = 172). In Group 4, RNA numbers coming from 18 slices in her1^ci301;her7^hu2526 (dark yellow, N = 2, n = 14, s = 494), her1^ci301;her7^hu2526;dlc^tw212b (black, N = 2, n = 16, s = 519), her1^ci301;her7^hu2526;dld^tr233 (purple, N = 3, n = 13, s = 442), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 2, n = 11, s = 366). Experimental, embryo, and slice data numbers are represented as N, n, and s, respectively. Significance test is performed in each group separately. The levels of significance are *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Exact p-values of the significance tests are put into Supplementary Table 3. The whisker plots show the median (line), quartiles (box), mean (plus sign), as well as the minimum and maximum (whiskers). Source data are provided as a Source Data file.