Fig. 3: DeltaC and DeltaD mediate most of the Notch activity in the PSM.

a Cartoon model showing how Compound E is blocking NICD cleavage by inhibiting gamma secretase (γ-Secretase) activity. Syringe symbol represents the application of Compound E drug, while the scissor symbol represents the γ-Secretase activity. b–e Transcription of her1 (cyan) (b, c) and her7 (red) (d, e) RNA in DMSO and Compound E treated her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos. Representative images are maximum intensity projections of a z-stack. The scale bar is 100 μm. f–h Histograms showing the percentage of cells with different her1 (f), her7 (g) and her1 plus her7 (total her) (h) RNA numbers in DMSO (gray, N = 2, n = 9, c = 14,000), and Compound E (orange, N = 2, n = 8, c = 9107) treated her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos. Experimental, embryo, and cell numbers are represented as N, n, and c, respectively. i her1 k her7 and m her1 plus her7 (total her) RNA numbers are plotted throughout the PSM (posterior to anterior) in her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos treated with DMSO (gray, N = 2, n = 9), and Compound E (orange, N = 2, n = 8). The average RNA number in each slice, along with 2 s.e.m., is represented by dots and error bars. j her1 l her,7 and n her1 plus her7 (total her) RNA numbers are compared in DMSO and Compound E treated embryos, by averaging all embryos in each group, as indicated in Fig. 2a. j, l, n In Group 1, RNA numbers coming from 10 slices in her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos treated with DMSO (gray, N = 2, n = 9, s = 150), and Compound E (orange, N = 2, n = 8, s = 119). In Group 2, RNA numbers coming from 20 slices in her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos treated with DMSO (gray, N = 2, n = 9, s = 355), and Compound E (orange, N = 2, n = 8, s = 308). In Group 3, RNA numbers coming from 8 slices in her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos treated with DMSO (gray, N = 2, n = 9, s = 144), and Compound E (orange, N = 2, n = 8, s = 127). In Group 4, RNA numbers coming from 16 slices in her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 embryos treated with DMSO (gray, N = 2, n = 9, s = 288), and Compound E (orange, N = 2, n = 8, s = 253). Experimental, embryo, and slice data numbers are represented as N, n, and s, respectively. Significance test is performed in each group separately. The levels of significance are *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Exact p-values of the significance tests are put into Supplementary Table 3. The whisker plots show the median (line), quartiles (box), mean (plus sign), as well as the minimum and maximum (whiskers). Source data are provided as a Source Data file.