Fig. 5: Notch signaling promotes deltaD transcription.

a, b Transcription of deltaD (red) RNA in her1^ci301;her7^hu2526 and her1^ci301;her7^hu2526; dlc^tw212b;dld^tr233 embryos. Representative images are single section of a z-stack. The scale bar is 100 μm. c deltaD RNA number per cell in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7, c = 8959), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10, c = 12313) embryos. Experimental, embryo, and cell numbers are represented as N, n, and c, respectively. d deltaD RNA number is plotted throughout the PSM (posterior to anterior) in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10) embryos. The average RNA number in each slice represented as dots, mean data, and 2 s.e.m are used in the graph. e Comparison of the deltaD RNA number across two genotypes, by averaging all embryos in each group, as indicated in Fig. 2a. In Group 1, RNA numbers coming from 10 slices in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7, s = 102), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10, s = 182). In Group 2, RNA numbers coming from 20 slices in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7, s = 254), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10, s = 387). In Group 3, RNA numbers coming from 8 slices in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7, s = 110), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10, s = 160). In Group 4, RNA numbers coming from 18 slices in her1^ci301;her7^hu2526 (dark yellow, N = 1, n = 7, s = 241), and her1^ci301;her7^hu2526;dlc^tw212b;dld^tr233 (green, N = 1, n = 10, s = 303). Experimental, embryo, and slice data numbers are represented as N, n, and s, respectively. Significance test is performed in each group separately. The levels of significance are *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Exact p-values of the significance tests are put into Supplementary Table 3. The whisker plots show the median (line), quartiles (box), mean (plus sign), as well as the minimum and maximum (whiskers). f, g Transcription of deltaD (red) RNA in DMSO and Compound E treated her1^ci301;her7^hu2526 embryos. Representative images are single section of a z-stack. The scale bar is 100 μm. h Histogram showing the percentage of cells with different numbers of deltaD RNA in DMSO (gray, N = 1, n = 12, c = 27947), and Compound E (orange, N = 1, n = 10, c = 23007) treated her1^ci301;her7^hu2526 embryos. Experimental, embryo, and cell numbers are represented as N, n and c, respectively. i deltaD RNA number is plotted throughout the PSM (posterior to anterior) in DMSO (gray, N = 1, n = 12), and Compound E (orange, N = 1, n = 10) treated her1^ci301;her7^hu2526 embryos. The average RNA number in each slice represented as dots, mean data, and 2 s.e.m are used in the graph. j, Comparison of the deltaD RNA number between two conditions, by averaging all embryos in each group, as indicated in Fig. 2a. In Group 1, RNA numbers coming from 10 slices in her1^ci301;her7^hu2526 embryos treated with DMSO (gray, N = 1, n = 12, s = 220), and Compound E (orange, N = 1, n = 10, s = 186). In Group 2, RNA numbers coming from 20 slices in her1^ci301;her7^hu2526 embryos treated with DMSO (gray, N = 1, n = 12, s = 480), and Compound E (orange, N = 1, n = 10, s = 400). In Group 3, RNA numbers coming from 8 slices in her1^ci301;her7^hu2526 embryos treated with DMSO (gray, N = 1, n = 12, s = 192), and Compound E (orange, N = 1, n = 10, s = 160). In Group 4, RNA numbers coming from 18 slices in her1^ci301;her7^hu2526 embryos treated with DMSO (gray, N = 1, n = 12, s = 429), and Compound E (orange, N = 1, n = 10, s = 360). Experimental, embryo, and slice data numbers are represented as N, n, and s, respectively. Significance test is performed in each group separately. The levels of significance are *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Exact p-values of the significance tests are put into Supplementary Table 3. The whisker plots show the median (line), quartiles (box), mean (plus sign), as well as the minimum and maximum (whiskers). k Cartoon model showing the currently identified intercellular positive feedback loop for both deltaC and deltaD. Source data are provided as a Source Data file.