Fig. 3: Le Dantec virus (LDV).

a Coverage plots for LDV identified by mNGS showing number of mapped reads (y-axis) at each nucleotide position across the genome (x-axis) for each virus. The annotated reference genome below plot shows LDV genes: nucleoprotein (N), phosphoprotein (P), matrix (M), glycoprotein (G), accessory gene (U1), polymerase (L). b ELISA analysis showing IgG antibody response to recombinant LDV glycoprotein in patient 220-2, contact of the patient and a healthy individual from Uganda. The plasmid containing an Ephrin β2 insert was used as a mock recombinant protein. The plot shows mean absorbance OD values at 450 nm (y-axis) and standard deviation (error bars) against increasing serum dilutions (x-axis) for n = 3 technical replicates from one representative experiment. c Pseudotype neutralisation assay showing luciferase reporter activity (y-axis) against increasing serum dilutions (x-axis) for patient 220-2, contact and healthy serum using LDV pseudotype virus compared with VSV control. Data shows the mean and standard error of mean for n = 3 technical replicates from one representative experiment. d Full genome maximum-likelihood phylogenetic nucleotide tree using the GTR + F + R4 model for Ledantevirus sequences illustrating the reported host for each virus. Evolutionary scale and ultrafast bootstrap values >90 are shown. The patient sample is highlighted in grey. Species silhouettes obtained through Phylopic; tick, midge, boar, bat fly, rodent, louse fly https://creativecommons.org/publicdomain/zero/1.0/, mosquito https://creativecommons.org/publicdomain/mark/1.0/.