Fig. 2: Regulatory B lymphocytes are not induced in mice after CLP.
From: PD-L1+ plasma cells suppress T lymphocyte responses in patients with sepsis and mouse sepsis models
a, b B splenocytes counts and percentages in 19 Sham and 24 CLP mice. c Proportion of CD1dhighCD5+ B splenocytes in 5 Sham and 6 CLP mice. d CD40, CD86 and MHC class II expressions as median fluorescence intensity (MFI) on splenic B cells from 6 Sham and 10 CLP mice. e PD-1, PD-L1, TIM-3 and LAG-3 expressions as MFI on splenic B cells (n = 5 Sham and 7 CLP mice for all markers except for LAG-3 where n = 11 sham and 9 CLP). f Volcano plot showing fold changes of 540 mRNA expressions in splenic B cells from 6 Sham and 6 CLP mice. Significantly upregulated mRNA are shown in red and downregulated ones in blue. g Splenic T cells from Sham mice (n = 8) were co-cultured with or without Sham (n = 16) or CLP (n = 16) purified splenic B cells at 1:1 ratio for 72 h, in the presence or absence of anti-CD3/CD28 antibodies-coated beads. T-cell proliferation was measured among viable cells by flow cytometry. Red squares represent results in CLP mice and white circles in Sham animals. h IFNγ was measured in culture supernatants of Sham T cells cultured either alone (n = 5) or with B cells from Sham (n = 7) or CLP (n = 12) mice. No IFNγ was detected in NS wells. Geometric means with standard deviations (SD) and individual values are shown. Two-sided Mann–Whitney tests were performed, and only significant p values < 0.05 are shown.
