Fig. 1: PGRMC2 was expressed in both human and mouse models, and metabolic acidosis was observed in hypoxic KO mice.
From: PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice
a IHC staining of PGRMC2 receptor in heart tissues of WT and KO mouse models. b Western Blot analyses of PGRMC2 and GAPDH in heart tissues of WT and KO mouse models (blue arrow heads). c Quantification of PGRMC2 expressions in heart tissues of WT and KO mouse models using two-tailed t-test. The protein expression of PGRMC2 was normalized with the GAPDH of the corresponding samples (n = 4 each; p = 0.0064). Blood gas analyses of pH (d; p = 0.0084 & 0.0006), pCO2 (e), BEecf (f; p = 0.0004 & 0.0005), and HCO3 (g; p = 0.0309, 0.0007 & <0.0001) were measured in WT and KO of normoxic or hypoxic mouse models. Blood hemoglobin (Hgb; h; p < 0.0001) and hematocrit (Hct; i; p < 0.0001) concentrations were also measured using i-STAT CG8+ cartridge. n = 6-12 for all data sets, n represents the number of mice. Statistical analysis of blood test is based on one-way ANOVA multiple comparisons, followed by Tukey’s post-hoc. Statistical significance between different groups is indicated in the graph with asterisk (*); data are presented as mean values ± SEM. Source data are provided in a Source Data file.
