Fig. 6: KO mouse cardiomyocytes were not responsive to estradiol, progesterone, and hydrocortisone.
From: PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice
a Representatives of 340 nm/380 nm excitation ratio using Fura-2AM test in WT, KO, and rescued cardiomyocytes were shown with treatment of estradiol (15 ng/mL), progesterone (40 ng/mL), and hydrocortisone (10 μg/mL) after baseline calcium level was recorded for 2–3 min. Ionomycin (5 μM) was used as a positive control. b–d Cytosolic calcium was quantified and compared between WT and KO cardiomyocytes in normoxic (b), hypoxic (c), and after rescued (d). n = 3–9 represents the number of cardiomyocytes (or independent experiments) from a total of 3 mice in each group. Statistical analysis is based on two-tailed t-test. Statistical significance between different groups is indicated with asterisk (*); *p = 0.025; **p = 0.0053; ***p = 0.0001; ****p < 0.0001; data are presented as mean values ± SEM. Source data are provided in a Source Data file.
