Fig. 4: Ksg, Ede, GE stabilize 30S initiation intermediates and inhibit of 70S-IC formation. | Nature Communications

Fig. 4: Ksg, Ede, GE stabilize 30S initiation intermediates and inhibit of 70S-IC formation.

From: The translation inhibitors kasugamycin, edeine and GE81112 target distinct steps during 30S initiation complex formation

Fig. 4

Refined maps of 30S-IF1-IF3-mRNA-tRNA complexes, with IF1 (red), IF3 (blue), fMet-tRNAfMet (light sea green) labelled bound to Ksg (a), Ede (b), and GE (c). d Overlay of mRNA path of GE-30S-IF1-IF3-mRNA-tRNA complex (green) with mRNA path of the 30S-PIC (closed form; turquoise; PDB ID 5LMU)6. A clash (red) of GE with mRNA path shows the flip of bases at -2 and -3 positions. e Overlay of tRNA and IF3 from our GE-30S-IF1-IF3-mRNA-tRNA complex with tRNA from 30S-IC (PDB ID 6O7K)7. The fMet-tRNAfMet of 30S-IC clashes with IF3 of our structure. f Alignment of GE-30S-IF1-IF3-mRNA-tRNA complex with 70S-IC (PDB ID 6O9K)7. IF3 of our structure clashes with H69 of 23S rRNA of 70S IC. g 70S IC formation by 50S binding to 30S ICs, as measured by light scattering. h IF3DL dissociation measured by FRET upon mixing 30S-IF3DL complexes with unlabeled IF3 using a stopped-flow apparatus. Buffer control traces indicate signal controls in the absence of the 50S (h) or unlabeled IF3 (g). Source data are provided as a Source Data file.

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