Fig. 3: POLQ/HELQ-mediated INDEL mutagenesis at psoralen ICLs. | Nature Communications

Fig. 3: POLQ/HELQ-mediated INDEL mutagenesis at psoralen ICLs.

From: FAN1-mediated translesion synthesis and POLQ/HELQ-mediated end joining generate interstrand crosslink-induced mutations

Fig. 3

A Tornado plots and B bar graphs representing the mutational spectrum for wild-type (n = 5), polq-1 (n = 2), helq-1 (n = 3) and polq-1 helq-1 (n = 2) animals. Data are presented as mean values ± SD (for n > 2). C Bar graph depicting the length of microhomology observed at the deletion junctions, and their contribution to the deletion spectrum of the indicated genotype: wild-type (n = 5), polq-1 (n = 2), helq-1 (n = 3), and polq-1 helq-1 (n = 2) animals. Data are presented as mean values ± SD (for n > 2). D Graphical representation of HELQ-dependent deletions either having 10 bp of perfect sequence homology or having 10 bp of sequence homology plus one non-matching nucleotide. Microhomology is highlighted in blue; the 1 bp mismatch is in bold and underlined. Bar graph shows the fraction of each outcome relative to the total deletion frequency for the indicated genotypes: wild-type (n = 5), polq-1 (n = 2), helq-1 (n = 3) animals. Data are presented as mean values ± SD (for n > 2). Source data are provided as a Source Data file.

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