Fig. 5: Dehydrocostus Lactone inhibits TMEM16A current in the TMEM16A recombinant HEK293T cells in a concentration-dependent manner.
A Voltage stimulation waveform scheme. B Representative TMEM16A current in whole-cell patch clamp in HEK293T recombination system is activated by 600 nM Ca2+ and inhibited by different concentrations of Dehydrocostus Lactone (n = 3), with 20 μM CaCCinh-A01 as the positive control. C I–V curve of TMEM16A inhibited by various concentrations of dehydrocostus lactone (n = 3), with three independent biological replicates performed for each group. Data are presented as mean values  ± SD. D Dose–response curve of TMEM16A currents at +80 mV incubated with indicated concentrations of dehydrocostus lactone (n = 3), with three independent biological replicates performed for each group. The IC50 of dehydrocostus lactone on TMEM16A channels was 111.97 ± 22.96 nM. Data are presented as mean values  ± SD. E Representative TMEM16A current in outside-out patch-clamp in HEK293T recombination system, which is activated by 600 nM Ca2+ and inhibited by 100 μM dehydrocostus lactone (n = 3), with 20 μM CaCCinh-A01 as the positive control. F I–V curve of TMEM16A inhibited by 100 μM dehydrocostus lactone (n = 3), with 20 μM CaCCinh-A01. n = 3 for each group, with three independent biological replicates performed for each group. Data are presented as mean values  ± SD.
