Fig. 1: Hachiman system provides defense against diverse phages. | Nature Communications

Fig. 1: Hachiman system provides defense against diverse phages.

From: Bacterial Hachiman complex executes DNA cleavage for antiphage defense

Fig. 1: Hachiman system provides defense against diverse phages.The alternative text for this image may have been generated using AI.

a Schematic of the Hachiman system gene cassette from Escherichia coli strain K-12. b Anti-phage defense phenotype of E. coli strain MG1655 (ΔhamAB) expressing Hachiman system with a native promoter. Fold defense was measured through serial dilution spot test. Each assay was performed with two replicates (detailed data in Supplementary Fig. 1). Growth curves for E. coli MG1655 strain expressing the Hachiman system or an empty vector, infected with phages T1(c) or phage TP041 (d) at an MOI of 0, 0.02, or 2. Data are mean ± SD of three technical replicates and are representative of two independent experiments. e Domain organization of HamA and HamB proteins of Hachiman system from the Escherichia coli str. K-12 genome. f Cryo-EM density map of the HamAB complex. g Cartoon of structural model of the HamAB complex. Blue, HamA-Cap4 domain; Green, HamA-DUF1837; Purple, HamB-NTD; Brown, RecA-1 domain; Yellow, RecA-2; Cyan, HamB-C helical.

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