Fig. 10: Selective chemogenetic activation or ablation of ZImPde11a neurons bidirectionally regulate sleep-wakefulness cycle. | Nature Communications

Fig. 10: Selective chemogenetic activation or ablation of ZImPde11a neurons bidirectionally regulate sleep-wakefulness cycle.

From: Transcriptomic and spatial GABAergic neuron subtypes in zona incerta mediate distinct innate behaviors

Fig. 10: Selective chemogenetic activation or ablation of ZImPde11a neurons bidirectionally regulate sleep-wakefulness cycle.

a Schematic diagram and representative images showing the injection of AAV-DIO-hM3Dq-mCherry or AAV-DIO-mCherry into the ZIm of Pde11a-Cre mice. Scale bar, 250μm. The experiment was independently repeated 10 times with similar results. b Schematic showing sleep recording conditions with saline- or CNO-injection in hM3Dq-group mice. c–e The percentage of time spent in wake (c ZT7, ***P = 0.0008), NREM sleep (d ZT7, ***P = 0.0007) and REM sleep (e ZT7, *P = 0.0117) in 2-h bins following injection of saline or CNO in hM3Dq-group mice. Wake, **P = 0.0056 (c); NREM, **P = 0.0080 (d); REM, **P = 0.009 (e). f–h Time spent in wake (f), NREM sleep (g) and REM sleep (h) across the 24-hour sleep-wake cycle in hM3Dq-group mice. Wake, **P = 0.0083 (f); NREM, *P = 0.0180 (g); REM, *P = 0.0304 (h). i Number and duration (j) of wake, NREM sleep and REM sleep episodes during the light phase. Number: Wake, **P = 0.0027; NREM, **P = 0.0026. Duration: NREM, ****P < 0.0001. k Schematic diagram showing the injection of AAV-DIO-DTA to ablate the ZImPde11a neurons. l–n The percentage of time spent in wake (l ZT21, *P = 0.0165), NREM sleep (m ZT21, *P = 0.0244) and REM sleep (n ZT21, *P = 0.0427) in 2-h bins in DTA-group mice compared with mCherry-group mice. REM, **P = 0.0040 (n). o–q Time spent in wake (o), NREM sleep (p) and REM sleep (q) across the 24-hour sleep-wake cycle in DTA-group mice compared with mCherry-group mice. Wake, **P = 0.0045 (o); NREM, **P = 0.0082 (p); REM, **P = 0.0013 (q). r Number and duration (s) of wake, NREM sleep and REM sleep episodes during the dark phase. Number: Wake, ***P = 0.0004; NREM, ***P = 0.0005; REM, **P = 0.0016. Duration: Wake, ***P = 0.0004. Two-way ANOVA and Sidak’s multiple comparisons test for (c-e) and (l-n). Two-tailed paired t test for (c–j). Two-tailed unpaired t test for (o–s). Data are presented as mean ± SEM. Gray bar indicates the dark phase (ZT12-ZT0). Source data are provided as a Source Data file.

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