Fig. 9: MTR4 depletion causes alternative splicing.
From: The nuclear exosome co-factor MTR4 shapes the transcriptome for meiotic initiation
A Pie plot showing the distribution of MTR4 binding reads mapped to the indicated regions of protein-coding genes. Categories are mutually exclusive. n = 1,081,322. B Bar plot showing the number of alternative splicing events in Mtr4-cKO germ cells compared to the corresponding Cntls. SE, skipped exons; RI, retained introns; MXE, mutually exclusive exons; A5SS, alternative 5’SS; A3SS, alternative 3’SS. C GO term analysis of the genes with significant SE events. P-values are based on hypergeometric test corrected for multiple hypothesis tests using the Benjamini-Hochberg method. D Sashimi plot of RNA-seq signals of altered SE event of Sycp1. E Sashimi plot of RNA-seq signals of altered SE event of Chfr. F RT-PCR analysis of altered SE events shown in (D, E). n = 3 biological repeats. G A model summarizing the role of MTR4 in shaping pre-meiotic transcriptome, ensuring proper meiosis initiation of germ cells and maintaining male fertility. Source data are provided as a Source Data file.
