Fig. 3: Transcriptional heterogeneity shapes the adaptive capacity of the cells.
From: Transcriptional heterogeneity shapes stress-adaptive responses in yeast
a Louvain clustering and projection onto wild-type 15 min UMAP, using variable genes as an input to define subpopulations. Cells are colored according to the subpopulation and the number is shown on top. b Heatmap shows expression of subpopulation-specific genes from the upregulated marker genes for each subpopulation defined in (a). Yellow colors indicate high expression and purple colors indicate low expression. c UMAP projection shows subpopulation representative genes with magenta for high expression and gray for low expression. d Distribution of the osmoconsensus signature expression induced for the indicated clusters. Solid black lines indicate the mean expression of the cluster while dashed black line indicates the population mean. Two-sided Wilcoxon test Benjamini–Hochberg adjustment of each cluster against the population is shown above each cluster. e Dot plot of 12 osmostress transcription factor activity across clusters. Dot size reflects the percentage of expressing cells; color indicates expression level (red = high, blue = low). f Scatter plot represents the single-cell co-expression of STL1, ALD3, CTT1, HSP12, GRE1, HXT5 (y-axis) and the induced osmoconsensus signature (x-axis). Cells are colored according to the Seurat cluster and dot size represents the expression levels of HXT5 as a representative gene of the hyper-responsive population. g Scatter plot represents the average expression correlation for the genes in the induced osmosconsensus signature for hyper-responsive cells (y-axis) or the rest of the population. h Scatter plot of the percentage of cells expressing the induced osmoconsensus program for the hyper-responsive cells (y-axis) or the rest of the population (x-axis). Black line represents the line of equality. i Bar plot represents the cell growth of NaCl-sorted top 2% pHXT5-UbiM-mCherry-tHXT5-expressing cells (red) against NaCl-randomly sorted constitutive GFP-expressing wild-type cells. Cells were mixed at 1:1 ratio (time 0) and grown in the presence of 0.8 M NaCl for 48 h. The abundance of each population was determined by FACS. Stacked bar plots the mean and error bars the standard deviation (n = 3). Two sided t.test against the top population at t0 is shown. Symbols ns/*/**/***/**** represent p-values > 0.05, <0.05, <0.01, <0.001, <0.0001. Source data are provided as a Source Data file.
