Fig. 3: RPE with enhanced interfacial diffusion and CE.
a Digital photo of the diffusion of RhB from the aqueous phase to the cyclohexane phase. b Schematic showing the difference in diffusion rate between RPE and SPE. The oil phase is a mixture of cyclohexane and octanol. RhB represents Rhodamine B. c Left: absorbance of RhB in cyclohexane phase as a function of time; right: the absorbance ratios (ARPE/ASPE) of RhB in cyclohexane phase between RPE and SPE as a function of time. d Schematic hydrolysis of p-NPP into p-nitrophenol catalyzed by lipase in RPE. e, f Comparison of (e) kinetic curves and (f) SA between different PEs for the hydrolysis of p-NPP into p-nitrophenol. p-NPP: 100 mg mL−1. Data are expressed as mean ± standard deviation of n = 3 independent experiments. g Schematic oxidation of pyrogallol into purpurogallin catalyzed by hemoglobin. h, i Comparison of (h) kinetic curves and (i) SA between different PEs for the oxidation of pyrogallol into purpurogallin. Pyrogallol: 130 mM. Data are expressed as mean ± standard deviation of n = 3 independent experiments. The SA of lipase was the activity per unit amount of the enzyme (U mg–1) in the reactions. The activity (U) was defined as 1 μmol product produced within 1 min. To obtain the SA, all reactions were repeated at least three times.
