Fig. 6: ChIP-seq confirms on-target chromatin association and epigenetic modifications. | Nature Communications

Fig. 6: ChIP-seq confirms on-target chromatin association and epigenetic modifications.

From: Programmable mRNA therapeutics for controlled epigenomic modulation of single and multiplexed gene expression in diverse diseases

Fig. 6

A HA ChIP-seq (orange) identifies on-target EC-chromatin association at the CXCL8 promoter (top) for CXCL-EC1 and at the upstream cRE (bottom) for CXCL-EC2. H3K9me3 ChIP-seq shows an establishment of the inhibitory signature mark at each locus after EC treatment (red) that is absent in control stimulated, untreated cells (black). B CXCL-EC1 and CXCL-EC2 treatment leads to a depletion of NF-kB binding at the CXCL8 promoter (top) and cRE (bottom) targets after stimulation (purple). Results are from 2 biological replicates from independent experiments.

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