Fig. 7: Astrocytic PTN within dmPFC interacts with PTPRZ1 in excitatory neurons to mediate antidepressant effects. | Nature Communications

Fig. 7: Astrocytic PTN within dmPFC interacts with PTPRZ1 in excitatory neurons to mediate antidepressant effects.

From: Astrocytic pleiotrophin deficiency in the prefrontal cortex contributes to stress-induced depressive-like responses in male mice

Fig. 7: Astrocytic PTN within dmPFC interacts with PTPRZ1 in excitatory neurons to mediate antidepressant effects.The alternative text for this image may have been generated using AI.

A–C Quantification of PTPRZ1 mRNA (A) and protein (B) (n = 6 WT mice per group), and verification by IHC analysis (C) (n = 6 replicates). D Quantification of isolated neuronal PTPRZ1 (n = 6 WT mice per group). E Schematic of the experimental diagram (WT mice) and IHC showing PTPRZ1 (green) co-expressed with CaMKIIα + (red) neuron (n = 6 replicates). F, G Validation of DIO-PTPRZ1-shRNA through quantification of PTPRZ1 mRNA (F) and protein (G) (n = 6 mice per group). H Behavioral tests analyses, including OFT, EPM, FST, TST, and SPT (n = 10 mice per group). I–K Traces of mEPSCs in CaMKIIα+ neurons (I), and quantification of amplitude (J) and frequency (K) (n = 30 cells from 10 mice per group). L Traces and quantification of action potentials in CaMKIIα+ neurons (n = 30 cells from 10 mice per group). M Traces and quantification of dendritic spines from CaMKIIα+ neurons (n = 30 cells from 10 mice per group). N Quantification of PSD95 (n = 6 mice per group). O Schematic of experimental design (WT mice). P Behavioral tests analyses, including OFT, EPM, FST, TST, and SPT (n = 10 mice per group). Q–S Traces of mEPSCs in CaMKIIα+ neurons (Q), and quantification of amplitude (R), and frequency (S) (n = 30 cells from 10 mice per group). T Traces and quantification of action potentials from CaMKIIα+ neurons (n = 30 cells from 10 mice per group). U Traces and quantification of dendritic spines from CaMKIIα+ neurons (n = 30 cells from 10 mice per group). V Quantification of PSD95 (n = 6 mice per group). Diagrams of the mouse brain and injection model in (E, O) were created in BioRender. chi, d. (2025) https://BioRender.com/x64m191. All data are presented as mean ± SEM. N.S. = not significant, *P < 0.05, **P < 0.01, ***P < 0.001. See Supplementary Data 5 for statistical details, including the statistical test used for data analysis and the exact P value. Source data are provided as a Source Data file.

Back to article page