Fig. 5: Schematic representation of the bacteriocin susceptibility assay.
From: Plasmid-driven strategies for clone success in Escherichia coli
A Description of bacteriocinogenic plasmids from pT 9-1 (ST131 clade B) and pT 10-1 (ST95). These archetypical pColV-like plasmids encode two bacteriocin gene clusters: microcin V and colicin Ia. Darker and lighter arrows indicate respectively the toxin (cvaC, cia) and immunity genes (cvi, iia) of each cluster. Control isolates either harbor no plasmid (ST131 clade B isolate 27-56) or harbor plasmid versions encoding only a single bacteriocin (colicin Ia for ST131 B isolate 31-16, microcin V for ST95 isolate 31-17). B Susceptibility of E. coli MG1655 to microcin V. Production of microcin V is induced by iron limitation (simulated experimentally with the addition of the chelating agent 2,2’-bipyridyl). Bacteriocinogenic activity is demonstrated by growth inhibition in the spotted area (in a dilution-dependent way and without formation of individual plaques). C Susceptibility of E. coli MG1655 to colicin Ia. Production of colicin Ia is induced through SOS induction (stimulated experimentally with UV irradiation). Bacteriocinogenic activity is demonstrated as in B. Created in BioRender. Gama, J. (2025) https://BioRender.com/e79b562.
