Fig. 6: N2O inhibits medium afterhyperpolarization potentials in L5 neurons.
a Mouse Cg1 coronal section immunostained for SK2 channel. SK2-positive cells are more prominent in L5 as opposed to L2/3. Scale bar, 20 μm. b Representative voltage traces of action potentials and medium afterhyperpolarization potentials (mAHPs) elicited by a 400 pA current injection under baseline conditions (black) and with 30% N2O (blue). Inset shows magnified mAHP as indicated by the teal box. c Summary data of mAHP amplitude across different current injection intensities (200–400 pA) under baseline (black) and 30% N2O (blue) conditions (n = 9 neurons from 4 mice). Two-way, repeated-measures ANOVA shows a main effect of N2O on the amplitude of mAHP (F(1,8) = 26.43, P < 0.001). Sidak’s post-hoc test results for individual current injections are shown in figure. d Representative control voltage traces under baseline conditions (black) and with 30% nitrogen (N2) (magenta). e Summary data of mAHP amplitudes across different current injection intensities under baseline (black) and 30% N2 (magenta) conditions (n = 7 neurons from 3 mice). Two-way ANOVA shows no effect of N2 on mAHP amplitude (F(1,6) = 0.279, P = 0.616), ns: P = 0.616. Data are presented as mean ± SEM.
