Fig. 1: Cryo-EM Structures of the 5-HT_2AR with Various Psychedelic Ligands.

All of the structures collected and analyzed in this work separated into the main ligand classes: tryptamines, ergolines, and phenethylamines. The local map, global map, ligand densities from the local map, and the resolution are given for each structure. All the maps shown were created from the half-maps utilizing deepEMhancer and the highRes model. However, the ligand densities are from the automatically sharpened output of the Local Refinement job in cryosparc. To maintain consistency, the receptor coordinates for both the global and local depositions remain identical and are modeled on the local refined map. All images were prepared using ChimeraX. The cartoon models shown in the bottom left represent all the receptors aligned to the 5-HT structure exhibiting the structural homogeneity between the ligands tested.