Fig. 5: PC abolishes the cytotoxic effect of CD8 T cells on MM cells by inhibiting GZMB expression through exosomal Sb9 derived from MM cells via LPA. | Nature Communications

Fig. 5: PC abolishes the cytotoxic effect of CD8 T cells on MM cells by inhibiting GZMB expression through exosomal Sb9 derived from MM cells via LPA.

From: Microbiota-reprogrammed phosphatidylcholine inactivates cytotoxic CD8 T cells through UFMylation via exosomal SerpinB9 in multiple myeloma

Fig. 5: PC abolishes the cytotoxic effect of CD8 T cells on MM cells by inhibiting GZMB expression through exosomal Sb9 derived from MM cells via LPA.The alternative text for this image may have been generated using AI.

a Level of exosomal Sb9 derived from KAS-6/1 and U266 cells treated with or without PC and LPA. Mean± SD, n = 3 biological replicates, data represent two independent experiments. b Representative images of the flow cytometric analysis for CD8 T cells cultured with PKH67-labeled exosomes (green) derived from WT or Sb9 KO KAS-6/1 and U266 cells treated with or without PC and LPA. c Sb9 protein levels in CD8 T cells cultured with PKH67-labeled exosomes (green) derived from WT or Sb9 KO KAS-6/1 and U266 cells treated with or without PC and LPA. Mean± SD, n = 3 biological replicates, data represent two independent experiments. d GZMB mRNA levels in CD8 T cells cultured with exosomes derived from WT or Sb9 KO KAS-6/1 and U266 cells treated with or without PC and LPA. Mean± SD, n = 3 biological replicates, data represent two independent experiments. e Released GZMB level in CD8 T cells cultured with exosomes derived from WT or Sb9 KO KAS-6/1 and U266 cells treated with or without PC and LPA. Mean± SD, n = 3 biological replicates, data represent two independent experiments. f Representative images of the flow cytometric analysis and quantification of the rate of RFP-labeled KAS-6/1 and U266 cells co-cultured with WT or Sb9-overexpressed CD8 T cells. Mean± SD, n = 3 biological replicates, data represent two independent experiments. Sb9 SerpinB9, PC phosphatidylcholine, LPA lysophosphatidic acid, GZMB granzyme B, KO knockout, OE overexpression, WT wild type. All statistical tests were two-sided. Statistical significance was assessed using one-way ANOVA followed by post-hoc Tukey’s test except for (b). Adjustments were made for multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Source data are provided as a Source Data file.

Back to article page