Fig. 3: Lipid chain length selectivity.
From: Structural insights into lipid chain-length selectivity and allosteric regulation of FFA2
a, b Cross-section (left) and surface representation (center and right) of FFA2 (a) and FFA124 (PDB ID: 8EJC) (b). c Superimposed images of FFA2 bound to TUG-1375 (purple, orange) and FFA1 bound to TAK-875 (green, yellow) focused on the orthosteric site. d–f Structural comparison around FFA1’s ligand entry site among active FFA2 (d), inactive FFA2 (e), and active FFA1 (f). g Mutagenesis analysis of the FFA2 mutant with a ligand entry site mimicking that of FFA1 (F873.30A/T1424.58G/I1454.61A, magenta) using the TGFα shedding assay upon stimulation with palmitic acid (a long-chain fatty acid). Symbols and error bars represent the mean and SEM, respectively, of 3 independent experiments, each performed in duplicate.
