Fig. 2: Distinct distribution patterns of Kremen1+ SPNs in the dorsal striatum and their projections.
From: Molecularly distinct striatonigral neuron subtypes differentially regulate locomotion
a Representative images of tdTomato (red) MOR1 (green) in the dorsal striatum of Kremen12A-Cre;Ai14 mice. dStr: dorsal striatum. vStr: ventral striatum. Scale bar: 1 mm. b Enlarged images of the boxed region in (a). Arrow points to pericytes that also express tdTomato. Scale bar: 200 µm. c Sample images of a sagittal brain section from a representative Kremen12A-Cre;Ai14 mouse. The tdTomato signals are prominent in the patch compartments of Str, GPe, EPN, SN and hippocampus (HIP). Scale bar: 2 mm. d Representative images of the SN area, showing the connection of tdTomato-positive SPN axon terminals with the dendrites of dopaminergic neurons in the SNr, forming the so-called dendron-bouquet structure. Bottom panel shows the magnification of boxed area from the top. Scale bars: 500 μm (top panel), 50 μm (bottom panel). e Schematics of AAV9-FLEX-tdTomato-synEGFP injection in the dorsal striatum of Kremen12A-Cre mice. The red vertical lines indicate the location of coronal sections in (f–h). f–h Images of tdTomato (red), sypEGFP (green) and TH (blue) staining in dorsal striatum (dStr), globus pallidus externus (GPe), entopeduncular nucleus (EPN), and substantia nigra (SN). Scale bars: 500 µm in dStr, 200 µm in GPe and EPN, 100 µm in SN.
