Fig. 3: Spontaneous network activity mirrors the learned interval-specific network dynamics.
From: Ex vivo cortical circuits learn to predict and spontaneously replay temporal patterns
A Sample traces of aligned spontaneous network activity (10 spontaneous events per cell and mean (bold)) from Opsin- pyramidal neurons in Early (purple) and Late (green) trained slices. B Comparison of the mean ± SEM (shading) of spontaneous activity in pyramidal neurons from Early (Opsin-: 18, ChR2+: 6) vs Late (Opsin-: 18, ChR2+: 3) trained slices. C Cumulative distribution of the spontaneous event peak times in ChR2+ and Opsin- pyramidal neurons from Early- vs Late-trained slices; p < 10−6, Kolmogorov-Smirnov test. D Normalized voltagegram of spontaneous events from recorded pyramidal neurons sorted by peak time in Early (left) vs Late (right) trained slices. E Average median event times of spontaneous events was significantly lower in neurons from Early- vs Late-trained slices. Opsin- neurons are shown in dark purple or dark green, while ChR2+ neurons are shown in light purple or light green. Data are presented as median event times ± SEM: 155 ± 26 ms and 427 ± 27 ms for Early and Late, respectively; U = 36, nEarly = 24, nLate = 21, p < 10−7, two-sided Mann–Whitney test. F Average median peak times of spontaneous events was significantly lower in neurons from Early- vs Late-trained slices. Data are presented as median peak times ± SEM: 54 ± 9 ms and 462 ± 42 ms for Early and Late, respectively; U = 11, nEarly = 24, nLate = 21, p = 10−10, two-sided Mann–Whitney test. G Mean center of gravity of spontaneous events was significantly lower in neurons from Early- vs Late-trained slices. Data are presented as mean center of gravity ± SEM: 230 ± 12 ms and 483 ± 13 ms for Early and Late, respectively; U = 3, nEarly = 24, nLate = 21, p < 10−10, two-sided Mann–Whitney test.
