Fig. 3: Antigen-specific systemic and mucosal antibody responses induced by intranasal (I.N.) immunization of the FPB NC vaccine. | Nature Communications

Fig. 3: Antigen-specific systemic and mucosal antibody responses induced by intranasal (I.N.) immunization of the FPB NC vaccine.

From: A combined adjuvant and ferritin nanocage based mucosal vaccine against Streptococcus pneumoniae induces protective immune responses in a murine model

Fig. 3: Antigen-specific systemic and mucosal antibody responses induced by intranasal (I.N.) immunization of the FPB NC vaccine.

A Experimental schedule for immunization. BALB/c mice were intranasally (I.N.) immunized with either phosphate-buffered saline (PBS), 6.5 μg of FlaB-tPspA, or 14.5 μg of FPB NC three times at two-week intervals for a total volume of 20 µl per mouse. B Determination of tPspA-specific systemic IgG or IgA titers. Two weeks after the final immunization, the levels of tPspA-specific serum IgG were evaluated using the enzyme-linked immunosorbent assay (ELISA). C Determination of the tPspA-specific mucosal IgA titer. Two weeks after the final immunization, tPspA-specific serum IgA levels in BAL-F, nasal wash, or saliva were evaluated using ELISA (n = 10 biological replicates). Data are presented as mean values ± SEM. Statistical differences were analyzed using one-way ANOVA. Statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns = not significant. Source data are provided as a Source Data file. Detail P values are provided in the Source Data file.

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