Fig. 7: The follicular helper T cell (Tfh) expansion in the draining lymph nodes by intranasal (I.N.) immunization of the FPB NC vaccine. | Nature Communications

Fig. 7: The follicular helper T cell (Tfh) expansion in the draining lymph nodes by intranasal (I.N.) immunization of the FPB NC vaccine.

From: A combined adjuvant and ferritin nanocage based mucosal vaccine against Streptococcus pneumoniae induces protective immune responses in a murine model

Fig. 7: The follicular helper T cell (Tfh) expansion in the draining lymph nodes by intranasal (I.N.) immunization of the FPB NC vaccine.

A Experimental schedule for immunization. BALB/c mice were intranasally (I.N.) immunized with PBS, 6.5 μg of FlaB-tPspA, or 14.5 μg of FPB NC two times at two-week intervals for a total volume of 20 µl per mouse. B Gating strategy to determine the percentage of \({{{\rm{PD-}}}}1^{+}{{{\rm{CXCR}}}}5^{+}\) cells within \({{{\rm{CD}}}}3^{+}{{{\rm{CD}}}}4^{+}\) population in mice immunized with FPB NC, FlaB-tPspA and PBS. C Detection of Tfhs by flow cytometry. Two weeks after the last administration, cLNs cells were prepared, and flow cytometric analysis was performed (n = 6 biological replicates). Data are presented as mean values ± SEM. Statistical differences were analyzed using one-way ANOVA. Statistical significance: *P < 0.05; **P < 0.01; ****P < 0.0001; ns = not significant. Source data are provided as a Source Data file. Detail P values are provided in the Source Data file.

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