Fig. 7: In vivo therapeutic efficacy of UCNP-Cro/FA upon irradiation.
A The schedule of cancer treatment in vivo. Each group received an intravenous injection of various formulations followed by NIR irradiation (980 nm, 2 W/cm2, 10 min) in those light-involved groups. Created in BioRender. Luwen, Z. (2025) BioRender.com/n87w615. B The tumor growth curves of mice under different treatments (n = 8 mice in each group). Statistical significance was calculated using an unpaired two-tailed t-test. C Tumor weights of mice at the end of different treatments (n = 3 mice in each group). Statistical significance was calculated using an unpaired two-tailed t-test. D Photos of excised tumors at the end of different treatments. E Survival rates of mice under different treatments (n = 5 mice in each group). F) Body weight curves of mice during the therapeutic period (n = 8 mice in each group). G Quantitative analysis of immunohistochemical staining for cleaved-caspase 1 (n = 3 mice in each group). Statistical significance was calculated using an unpaired two-tailed t-test. H Quantitative analysis of immunohistochemical staining for GPX4 (n = 3 mice in each group). Statistical significance was calculated using an unpaired two-tailed t-test. I MDA content in the tumor tissues after different treatments (n = 3 mice in each group). Statistical significance was calculated using an unpaired two-tailed t-test. J) Representative images of tumor slices from different groups for H&E staining, TUNEL staining, and immunohistochemical staining of GPX4 and cleaved-caspase 1. Scale bar=100 μm. Experiments were repeated three times independently with similar results. I: Control, II: NIR, III: UCNP-Cro/FA, IV: UCNP-FA + NIR, V: UCNP-Cro+NIR, VI: UCNP-Cro/FA + NIR. Error bars represent the mean ± SD. Source data are provided as a Source Data file.
