Fig. 2: Effect of TIC and sparse LOESS normalization on technical variance in MSI signal intensity.
From: Resolving multi-image spatial lipidomic responses to inhaled toxicants by machine learning
Total ion current scatterplots of signal intensity vs. pixel number according to acquisition order in negative ionization mode for (a) raw, (b) TIC, and (c) sparse LOESS normalized data. d–f Total ion current ion images for a representative sample displaying the raw, TIC, and sparse LOESS normalized signal intensity in negative mode, respectively. TIC values correspond to the sum intensity of all annotated compounds for each pixel. Sample ID reflects the identity of individual imaging runs in order of data acquisition. Bar = 1 mm. Source data for panels (a–c) are provided as Source Data files.
