Fig. 1: Mice explore and refine directional spatial target reaches.
From: Dissociable roles of distinct thalamic circuits in learning reaches to spatial targets
a Schematic of a head-fixed mouse performing the Spatial Target Task (STT) during imaging. b Left: top view schematic of the SCARA joystick. Start position (green circle) and example position of target 1 shown. Right: same as left, example of target 2 and previous target 1 (gray circle). c Classification of hit and miss trajectories from self-paced joystick movements. d Schematic showing training protocol. e Left: example trajectories on early and late days of block 1 with rewarded target (blue circle) and non-rewarding target (gray circle). Right: same for block 2 with rewarded target (magenta circle) and non-rewarding target (gray circle). Hit trajectories (yellow) and miss trajectories (gray) are plotted. Point of target entry and reward delivery is shown (blue dot). f Hit ratio on 5 selected days per block from early training day (E) to late training day (L) and 3 equidistant days in between. Mixed-effects model, day in block: F(3,25) = 40, *p < 0.0001, day x block: F(3,14) = 4, *p < 0.05. Å Ãdák’s multiple comparisons test: E vs L block 1, *p < 0.005; E vs L block 2, *p < 0.0001. g Left: representative heatmap of workspace occupancy of all trajectories of an early and late session. Right: occupancy of workspace (mm2). Mixed-effects model, day in block: F(3,23) = 13, *p < 0.0001, mouse: F(1,9) = 6, *p < 0.05. Å Ãdák’s multiple comparisons test: E vs L block 1, *p < 0.05; E vs L block 2, *p < 0.005. h Variability of mean trajectory from all movements. Mixed-effects model, day in block: F(2,20) = 19, *p < 0.0001, mouse: F(1,9) = 17, *p < 0.01. Å Ãdák’s multiple comparisons test: E vs L block 1, *p < 0.005; E vs L block 2, *p < 0.005. i Variability of initial direction of movement. Mixed-effects model, day in block: F(3,22) = 8, *p < 0.01, mouse: F(1,9) = 7, *p < 0.5. Å Ãdák’s multiple comparisons test: E vs L block 1, *p < 0.05; E vs L block 2, *p < 0.005. j Peak speed of trajectories. Mixed-effects model, day in block: F(4, 36) = 4, *p < 0.05, mouse: F(1,9) = 8, *p < 0.05. Å Ãdák’s multiple comparisons test: E vs L block 1, p > 0.05; E vs L block 2, *p < 0.05. k Average target overshoot from target entry for all hit trajectories. Mixed-effects model, F(3,25) = 8, *p < 0.001, mouse: F(1,9) = 9, *p < 0.05. Å Ãdák’s multiple comparisons test: E vs L block 1, p > 0.05; E vs L block 2, *p < 0.05. f–k Mean ± SEM is shown in thick color lines and shaded bounds (block 1: blue, block 2: magenta), single animals shown in gray lines (n = 10 mice). Asterisks indicate Å Ãdák’s multiple comparisons test for E vs L day in block. See also Supplementary Fig. 1. Source data are provided as a Source Data file.
