Fig. 5: Disruption of cell-cell contacts by α-catenin depletion abolishes ferroptosis propagation.
From: Ferroptosis spreads to neighboring cells via plasma membrane contacts
Opto-GPX4Deg and bystander HeLa (a–d), SCLC (e–h), or HT29 (i–l) cells transfected with either siRNA against α-catenin or scramble siRNA (control), and treated or not with 5 µM Fer-1. a, e, i Kinetics of cell death in α-catenin KD and control samples. b, f, j %AUC for indicated populations. Experiments were performed with three independent biological replicates (n = 3). c, g, k WB analysis of indicated protein levels in α-catenin KD and control cells. d, h, l Quantification of WB in (c, g, k). Protein levels normalized to loading control. Experiments were performed with three independent biological replicates (n = 3). m Kinetics of cell death in Opto-GPX4Deg and bystander HeLa cells transfected with RFP-tagged empty vector, WT E-cadherin, E-cadherin ΔCBD mutant, WT N-cadherin or WT P-cadherin. n %AUC for the cell populations in (m) Experiments were performed with three independent biological replicates (n = 3). Statistical analysis by two-sided one-way ANOVA corrected for multiple comparisons using Tukey’s multiple comparison test or by parametric t-test (d, h, l). Exact p values are shown. All experiments were performed with three independent biological replicates (n = 3). Values are displayed as mean ± SD.
