Fig. 2: Transplantation of liver-derived healthy mitochondria into the degenerated cerebellum of CBND mice and associated mitochondrial quality control. | Nature Communications

Fig. 2: Transplantation of liver-derived healthy mitochondria into the degenerated cerebellum of CBND mice and associated mitochondrial quality control.

From: Mitochondria transplantation transiently rescues cerebellar neurodegeneration improving mitochondrial function and reducing mitophagy in mice

Fig. 2: Transplantation of liver-derived healthy mitochondria into the degenerated cerebellum of CBND mice and associated mitochondrial quality control.

a Rapid isolation of the liver from a male C57BL/6J mouse. b Rapid isolation of mitochondria from the liver tissue. c Mitochondrial quality control assessed by Mitotracker Red staining. d, e Mitochondrial quality confirmation by Mitotracker observation under confocal microscope (Scale bar = 10 μm) (d) and Western Blot analysis of VDAC1 (n = 3 mice) (e). f Quantification of mitochondrial membrane potential levels using JC-1 fluorescence in isolated mitochondria at 0 or 1 h post-isolation. Two-sided Mann-Whitney U test; n = 16 (4 mice, 4 technical replicates). g Quantification of ROS levels using DCFH-DA fluorescence in isolated mitochondria at 0 or 1 h post-isolation. Two-sided Mann-Whitney U test, n = 9 (3 mice, 3 technical replicates). h The process of mitochondria transplantation into the cerebellum of CBNDgeneral mice. The data was shown by mean ± SD. Cartoon images were created in BioRender. Li, S. (2025) https://BioRender.com/j40j862. A anterior, CBND cerebellar neurodegeneration, Cb cerebellar lobule, h hour, L left, Mito mitochondria, P posterior, R right.

Back to article page