Fig. 7: Exogenous mitochondria support degenerated Purkinje cells by inhibiting Caspase3-related cell apoptosis and Parkin-related mitophagy.
a Q-PCR results of Drp1, SCA1, SCA6, SCA7, and SCA17 mRNA from cerebellum of CBNDgeneral mice tested at 1 month old. Two-tailed unpaired t test or Mann-Whitney U test, n = 6 (3 mice, 2 technical replicates). b Western blotting results of DRP1, DRP1S616, DRP1S637, FIS1, MFF, and SOD from cerebellum of CBNDgeneral mice tested at 1 month old. Two-tailed unpaired t test or Mann-Whitney U test, n = 5 mice. c Confocal images of Caspase3, Cox4, and Parkin (White) stained on Purkinje cells (Red) of CBNDTdToamto mice at 1-, 2-, 3-, and 4-month-old (left) or on mito-treated CBNDTdToamto mice (right) 3 weeks post-transplant. Bar = 5 μm. n = 3 mice. Quantification of Caspase3 (d), Cox4 (f), and Parkin (h) expressing on Purkinje cells of CBNDTdToamto mice at 1-, 2-, 3-, and 4-month-old. One-way ANOVA and Dunnett’s t test, n = 6 (3 mice, 2 technical replicates). Quantification of Caspase3 (e), Cox4 (g), and Parkin (i) expressing on Purkinje cells of mito-treated CBNDTdToamto mice (right) 3 weeks post-transplant. Two-tailed unpaired t test, n = 6 (3 mice, 2 technical replicates). The data was shown by mean ± SD. CBND cerebellar neurodegeneration, Mito mitochondria.
