Fig. 1: Biosynthetic pathway and structural architecture of AftB.
From: Structural insights into terminal arabinosylation of mycobacterial cell wall arabinan
a Schematic of the arabinogalactan biosynthetic pathway in mycobacteria, featuring the enzymatic reactions carried out by AraTs. Enzyme names are indicated above the reaction arrows. The inset shows the β-(1 → 2) arabinosyl transfer reaction catalyzed by AftB. The arabinan chain is shown linked to the 10th position of the galactan chain through a β-(1 → 2) bond. While this linkage also occurs at the 8th or 12th position, the 10th position is depicted here for clarity. b Cryo-EM density map of AftB in complex with Fab-B3. AftB is shown in blue, while Fab-B3 is shown in maroon. c Topological diagram of AftB showing the arrangement of secondary elements in the TM region and in the PD, annotated and colored in correspondence with (d). The catalytic residue D62 is denoted as a red dot. d Structure of AftB shown in ribbon. The unmodeled region of AftB is marked as dashed lines, and membrane boundaries are marked by dotted horizontal bars.
