Table 2 Amounts of the injected mRNAs and concentrations of morpholinos used in this work
RNA name | RNA, pg | cPMO, uM | tbMO, uM | Stage of irradiation, hpf | Irradiation, min |
|---|---|---|---|---|---|
tis-GFPnls (internal DB Nr F041) | 20 | 500 | 25 | 4 | 5 |
tis-kid (PCR template) | 5 | 500 | 50 | 10 | 7 |
tis-bmp2b (internal DB Nr F045) | 10 | 200 | 30 | 4-5 | 9 |
tis-ctnnb1 (PCR template) | 100 | 500 | 10 | 2-3 | 7 |
tis-cxcr7 (internal DB Nr F374) | 600 | 500 | 25 | 24 | 3 |
tis-control (internal DB Nr F374) | 300 | 500 | 25 | 24 | 3 |
