Fig. 2: Structure of the NS1/MED25 ACID complex.

A The 1:1 complex of NS1/MED25 ACID in ribbon representation with MED25 ACID oriented to show the “H2” binding site (left) and in a 180° rotated configuration to show the “H1” binding site (right). NS1, teal; MED25 ACID; violet. B Analysis of the NS1/MED25 ACID complex reveals extensive hydrophobic interactions and surface complementarity among residues at the interface. Electrostatic surface representation of MED25 ACID with NS1 in ribbon representation. Red, white, and blue represent negative, neutral, and positive electrostatic potential, respectively (−10 to +10 kBT e-1). Orientations are as in A.