Fig. 4: In vitro drug delivery characterization of MN and stability of the released drug.

A In vitro glucose-responsive release of Z-GCN from the MN patch in PBS mimicking hypoglycemia (50 mg/dL), euglycemia (100 mg/dL) or hyperglycemia (300 mg/dL) (n = 3 independent samples, mean ± SD). B Pulsatile release of Z-GCN from the MN patch in varying glucose media (50, 100 and 300 mg/dL) (n = 3 independent samples, mean ± SD). C CD spectra of native glucagon solution and released Z-GCN. D The deconvolution of CD spectra to reveal fractions of glucagon secondary structure. E The calculated interaction mode between Z-GCN and the polymer, as determined by clustering analysis. Z-GCN and polymer residues are shown as ball-and-stick model and wire representation.