Fig. 6: EBOV antagonizes JFB’s type I interferon pathway more efficiently than MARV.
From: Jamaican fruit bats’ competence for Ebola but not Marburg virus is driven by intrinsic differences
A Infectious titers of EBOV-Mayinga and MARV-Ozolin on Jamaican fruit bat kidney cells (AjKi_RML2) infected with multiplicity of infection (MOI) 0.1. Data presented as log10 transformed values. Data are from two independent experiments with three technical replicates each. Two-way ANOVA with Sidak’s multiple test comparison to evaluate the difference between EBOV and MARV titer at each time point. B Immunoblot of AjKi_RML2 cells infected with EBOV or MARV at MOI 0.1. The presented panel is representative of three independent immunoblots. RT-qPCR of IFNb1 (C), interferon stimulated gene (D), or pro-inflammatory cytokine (E) mRNA in AjKi_RML2 cells infected with EBOV or MARV at MOI 0.1. F Infectious titers of three EBOV and MARV strains on AjKi_RML2 cells infected with MOI 0.1. Data presented as log10 transformed values. Data are from one experiment conducted in triplicate. RT-qPCR of Infb1 (G), interferon stimulated gene (H), or pro-inflammatory cytokine (I) mRNA in AjKi_RML2 cells infected with three different EBOV or MARV strains at MOI 0.1. J Infectious titers of three EBOV and MARV strains on Jamaican fruit bat immortalized kidney (AJi), primary kidney (AjKi_RML1), primary lung (AjLu_RML3) infected with MOI 0.1. Data presented as log10 transformed values. Data are from one experiment conducted in triplicate. RT-qPCR of Ifnb1 (K), interferon stimulated gene (L), or Tnfa (M) mRNA in Jamaican fruit bat cells infected with three EBOV Mayinga or MARV Ozolin strains at MOI 0.1. C–E; G–I; K–M ΔCT values were normalized to the average ΔCT value of mock infected cells to calculate ΔΔCT. Data from three biological replicates. Fold change of each gene at each time point for EBOV and MARV-infected cells was compared to mock using a two-way ANOVA with Dunnett’s multiple test comparison. A, C–M Data plotted as mean ± S.D. A, C–E, G–I, K, L P values for comparisons to mock cells are indicated. **** <0.0001, *** <0.001, ** <0.01, * <0.05.
