Fig. 8: OS of Piezo2⁺ mechanical nociception promotes implant osseointegration by activation of CGRP-PKA-FAK signaling pathway.

a, b Micro-CT images and quantitative analysis of the bone tissue surrounding the implants after 2 weeks of treatment with different antagonists/inhibitors, scale bar: 100 μm. Data are presented as mean ± SEM (n  =  5 biologically independent experiments). P-values were calculated using one-way ANOVA with Tukey’s multiple comparisons test. c, d Expressin levels and semi-quantitative analysis of osteogenesis-related proteins (Runx2/ALP/OPN/OCN) in the bone tissue surrounding the implants after 2 weeks of treatment with different antagonists/inhibitors (The samples derive from the same experiment and that blots were processed in parallel). Data are presented as mean ± SEM (n  =  5 biologically independent experiments). P-values were calculated using one-way ANOVA with Tukey’s multiple comparisons test. (ALP: alkaline phosphatase, Runx2: recombinant Runt related transcription factor 2, OPN: osteopontin, OCN: osteocalcin, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, BV/TV: bone volume/total volume, Tb.Th: trabecular thickness, Tb.N: number of trabeculae, Tb.Sp: trabecular spacing, BMD of BV: bone mineral density of bone volume, BIC%: bone-implant contact percentage). Source data are provided as a Source Data file.