Fig. 3: The phosphorylation of hPhK.
From: Molecular basis for the regulation of human phosphorylase kinase by phosphorylation and Ca2+
a, b Representative annotated MS spectrum of the α (a) and β (b) subunits. c The phosphorylation level of hPhK. Phosphoprotein staining is used to detect phosphorylated proteins, while Coomassie Blue staining is used to detect total proteins. d The specific activities of dephosphorylated and phosphorylated hPhK at pH 6.8 and 8.2 in the presence of Ca2+ or EGTA. Data points represent the mean ± SD of four (Dephosphorylation) or five (phosphorylation) measurements.
