Fig. 3: Activation of endogenous genes using the Cas12f/cgRNA system. | Nature Communications

Fig. 3: Activation of endogenous genes using the Cas12f/cgRNA system.

From: Engineered circular guide RNAs enhance miniature CRISPR/Cas12f-based gene activation and adenine base editing

Fig. 3

a Targeted gene activation guided by Normal, linear, and circular gRNAs in the dCas12f-VPR knock-in (KI) HEK293T cell line transfected with 16 ng gRNAs. Data are presented as mean values ± SD. b Targeted gene activation guided by Normal, linear and circular gRNAs in MCF7, THP1, and MV4-11 cell lines transiently co-transfected with dCas12f-VPR and gRNA-encoded plasmids. Data are presented as mean values ± SD. c Specificity of gene activation by the Cas12f/cgRNA system. Gene expression profiles derived from RNA-seq data of KI HEK293T cells transfected with normal, linear, and circular gRNAs targeting HBG1, and cgRNAs targeting mNeonGreen (negative control, NC). For a–c, n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA test. Source data are provided as a Source Data file.

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