Fig. 4: Phase separation enhances the Cas12f/cgRNA system-induced gene activation. | Nature Communications

Fig. 4: Phase separation enhances the Cas12f/cgRNA system-induced gene activation.

From: Engineered circular guide RNAs enhance miniature CRISPR/Cas12f-based gene activation and adenine base editing

Fig. 4

a Schematic diagram of the phase-separated activation using the Cas12f/cgRNA system. b RT-PCR analysis showed relative mRNA expression of targeted gene in HEK293T cells utilizing the Cas12f/cgRNA system combined with phase separation system. Data are plotted as mean ± SD (n = 3 independent experiments), *p < 0.05, **p < 0.01, ***p < 0.001, two-sided Student’s t-test. c GFP fluorescence in HEK293T cells transfected with dCas12f-VPR-sfGFP or dCas12f-VPR-FUSIDR-sfGFP plasmid. n = 5 independent experiments, Scale bar, 5 μm. d The fluorescence recovery after photobleaching (FRAP) analysis of dCas12f-VPR-FUS-sfGFP droplets in HEK293T cells. Scale bar, 5 μm. Data are plotted as mean ± SD (n = 5 independent experiments). Source data are provided as a Source Data file.

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